Table II.

MLTC-derived CD4+ T cells proliferate in vitro in response to autologous DC pulsed with AWE from autologous but not allogeneic metastatic tumors

CD4+ T Cell Donorsa (patient no.)AWE Donors (patient no.)
12345678910
1 17.96b 0.950.850.54 9.26 0.391.010.35 5.87 0.24
20.71 12.91 0.320.671.350.561.700.921.950.76
31.521.71 15.76 0.790.850.921.630.751.070.93
40.630.350.97 10.59 1.321.250.761.230.690.91
5 5.35 0.520.440.32 11.52 0.390.620.53 6.30 0.65
60.220.950.550.780.51 10.25 0.420.400.430.44
71.721.950.620.760.392.10 21.23 0.951.250.35
80.520.230.170.650.720.350.17 8.90 1.900.35
9 6.25 0.720.830.69 9.65 0.520.720.70 19.87 0.71
101.052.010.720.360.640.570.620.920.76 13.55
  • a MLTC-activated CD4+ T cells were tested for proliferative responses in cultures with autologous DC pulsed with AWE from autologous or allogeneic metastatic tumors (see also Materials and Methods).

  • b Mean values from triplicate cultures are given as cpm × 10−3. The SD was always <15% of the mean values and thus is omitted. Values in bold indicate statistically significant (p < 0.01) proliferative responses over background values (i.e., CD4+ T cells with nonpulsed DC, which never exceeded 1200 cpm).