Table II.

Effect of different neo-glycoproteins, sugars, and matrix components on the phagocytosis of PSG by AMa

Inhibitor UsedInhibitor Concentration% Inhibition of PSG Phagocytosis
Man-BSA50 μg/ml65.4 ± 13.1*
Fuc-BSA50 μg/ml55.2 ± 3.2*
GalNAc-BSA50 μg/ml60.9 ± 7.7*
Gal-BSA50 μg/ml67.9 ± 4.9*
Gluc-BSA50 μg/ml55.2 ± 3.9*
BSA alone50 μg/ml0.0 ± 12.6
Sialic acid10 mM−15.9 ± 9.4
N-acetylglucosamine10 mM7.6 ± 8.5
Laminarin10 mg/ml−32.4 ± 7.6*
Mannan10 mg/ml7.0 ± 7.9
Fucoidan10 mg/ml21.6 ± 15.2
Laminan50 μg/ml1.4 ± 8.2
Fibronectin50 μg/ml23.1 ± 2.8
RGD-ser50 μg/ml36.2 ± 12.6*
  • a AM were incubated with inhibitor for 40 min prior to the addition of FITC-labeled rye grass PSG at an MOI of 40 for 4 h. Inhibition of phagocytosis was calculated using a control in which no inhibitor was present. Results show the mean percentage of inhibition of phagocytosis ± SEM (n = 3) and are representative data from three separate dose-response experiments.

  • b , p < 0.05, when comparing the level of phagocytosis in the presence of inhibitor to phagocytosis in the absence of inhibitor; i.e., an individual control was used for each inhibitor.