Table I.

Transduction efficiencies for Ag-activated PL/J and MBP TCR transgenic splenocyte populations

No. of SplenocytesaPercentage of MBP CellsExperimental Transduction EfficiencyObserved Transduction Efficiency
PL/JMBP
01× 106100∼50
1 × 10600<0.5
1 × 10610000.10.050.09
1 × 10610,0001.00.50.45
1 × 106100,00010.05.04.5
1 × 1061× 10650.025.020.4
1 × 1062× 10666.633.332.9
  • a Retroviral transduction was performed on naive splenocyte cultures from PL/J and/or MBP TCR transgenic mice. Expected transduction efficiencies were based on the observation of 50% transduction efficiency of Ag-activated MBP-specific splenocytes infected with retrovirus in the absence of PL/J splenocytes. Efficiencies of cells transduced are reported as percentages of CD4+/GFP+ double positive T cells.