Table I.

Effect of mAbs to chemokine receptors on TGF-β1-induced enhancement of chemotaxis of iDCs to RANTES and SDF-1αa

ChemoattractantmAbNo. of Migrated Cells (Mean ± S.D.), Treatment with
NoneTGF-β1
NoneNone6 ± 17 ± 1
RANTESNone48 ± 3107 ± 5
cIgG51 ± 3106 ± 5
Anti-CCR-1 mAb26 ± 453 ± 6
Anti-CCR-3 mAb34 ± 266 ± 4
Anti-CCR-5 mAb43 ± 284 ± 7
Anti-CCR-1 mAb/anti-CCR-3 mAb/anti-CCR-5 mAb15 ± 318 ± 4
Anti-CXCR 4 mAb50 ± 3110 ± 10
SDF-1αNone48 ± 4113 ± 6
cIgG51 ± 2115 ± 4
Anti-CCR-1 mAb49 ± 3117 ± 7
Anti-CCR-3 mAb48 ± 2115 ± 10
Anti-CCR-5 mAb50 ± 4116 ± 9
Anti-CXCR 4 mAb13 ± 320 ± 3
  • a iDCs were unstimulated or stimulated with TGF-β1 (10 ng/ml) for 3 days. These cells (106) were pretreated with stated mAbs (1 μg/ml) for 30 min at 37°C and seeded on the filters precoated on the lower surface with 5 μg gelatin. RANTES or SDF-1α (10 ng/ml) used as chemoattractants were added to the lower chamber. After a 2-h incubation, the migrated cells on the lower surface were visually counted.