Table III.

Both Mφ and MC produce NO in response to proinflammatory stimulia

Cell TypeControlNitrite Produced Over 24 hIl-1β
IFN-γ/LPSIFN-γ/TNF-α
Rat BMD Mφ5.6 ± 1.1125.3 ± 10.073.5 ± 13.4ND
Mouse BMD Mφ
129/sv wild type3.7 ± 0.4134.2 ± 38.2NDND
NOS-2−/−2.4 ± 0.432.1 ± 5.6NDND
Rat glomerular Mφ
Control animals15.7 ± 4.2ND100.7 ± 4.8ND
Day 2 NTN154.8 ± 2.5NDNDND
Day 4 NTN109.5 ± 2.3NDNDND
Rat mesangial cells
Clonalb1.2 ± 1.41.6 ± 2.023.4 ± 7.61.7 ± 2.0
Polyclonalb2.7 ± 2.14.5 ± 0.451.3 ± 15.73.9 ± 2.2
  • a Values are mean ± SE (n ≥ 4). BMD Mφ were either untreated or activated with IFN-γ (100 U/ml) plus TNF-α (100 U/ml) or IFN-γ (100 U/ml) plus LPS (1 μg/ml). Resident rat glomerular Mφ were untreated or activated with IFN-γ (100 U/ml) plus TNF-α (100 U/ml). Mφ from rat glomeruli with nephrotoxic nephritis on days 2 and 4 were untreated. MC were untreated or activated with IFN-γ (100 U/ml) plus TNF-α (100 U/ml), IFN-γ (100 U/ml) plus LPS (1 μg/ml), or Il-1β alone (40 ng/ml).

  • b Nitrite produced over 48 h. ND, not done.