Table II.

Effect of l-NMMA and d-NMMA on activated Mφ-induced induction of MC apoptosis and suppression of mitosis in rat MCa

l-NMMAd-NMMA
CocultureControlCocultureControl
% Apoptosis2.6 ± 1.22.9 ± 1.916.3 ± 4.3b3.5 ± 2.1
% Mitosis2.9 ± 0.22.3 ± 0.10.2 ± 0.2b2.6 ± 0.2
Cell number908 ± 104911 ± 86549 ± 78b820 ± 19
  • a Coculture was established as described in Materials and Methods. After priming with IFN-γ, Mφ were activated using IFN-γ (100 U/ml) and TNF-α (100 U/ml). Either d-NMMA (100 μM) or l-NMMA (100 μM) was added to wells. Experiments were assessed at 24 h. Data are given as mean ± SEM.

  • b , p < 0.05 compared with coculture in the presence of l-NMMA (n = 4).