Table I.

Effect of Mφ on the proportion of cells exhibiting DNA content of G2/M-phase of the cell cycle or hypodiploid DNA contenta

Unstimulated CocultureActivated CocultureActivated Coculture + l-NIL
% G2/M-phase cells18.1 ± 1.510.4 ± 1.4b20.9 ± 1.5
% Hypodiploidy0.6 ± 0.36.2 ± 2.2b1.0 ± 0.3
  • a Cells were prepared and analysed according to Materials and Methods with 1.0 MC:1.5 Mφ. Mφ were primed with IFN-γ (100 U/ml), and once coculture was established, activated with IFN-γ and LPS (1 μg/ml). Note data shown refer to whole coculture. MC controls were activated with the same agents and showed 19.2 ± 0.7% in G2/M, 0.3 ± 0.1% hypodiploidy. Activated Mφ alone demonstrated 0.3 ± 0.1% hypodiploidy. No Mφ had increased DNA content.

  • b , p < 0.05 compared with unstimulated coculture (n = 3).