Table II.

Major peptide fragments generated by 20S proteasome-mediated digestion of MuLV natural sequences

MuLV TypeSequenceDigestion Time (h)
1424
MoloneyTGRAYWKPSSSWDFITVNNNLTSDQA
SWDFITV45a,b6472
PSSSWDFITV233628
TGRAYWKPSSSWDFITV18
AYWKPSSSWDFITV14
AKVTGRASWKPSSSWDYITVSNNLTSDQA
TGRASWKPSSSWDYITVSNNLTSDQA57
ASWKPSSSWDYITVSNNLTSDQA29
TGRASWKPSSSWDYITV1412
SWDYITV4175
ASWKPSSSWDYITV30
PSSSWDYITV1725
FriendTGRAYWKPSSSWDYITVDNNLTTNQA
TGRAYWKPSSSWDYITVDNNLTTNQA60
TGRAYWKPSSSWDYITVD2125
AYWKPSSSWDYITVD820
AYWKPSSSWDYITVDNNLTTNQA6
PSSSWDYITVDNNLTTNQA5
SWDYITVD3569
PSSSWDYITVD2031
  • a Intensity is given as a percentage of the total amount of intensity of the peak fragments depicted below.

  • b Fragments with intensities less than three times the noise peak in a spectrum were ignored in the determination of sequences of peptides generated after digestion by 20S proteasomes.