Table III.

SIV epitope and protein-specific precursor frequencies in three sooty mangabeys determined by limiting dilution analysis

MangabeysTarget CellsaCTLP/ 1/x (95% CI values)bCTLP/106 PBMC (95% CI values)
FLgNYCBH>3,800,0000.3 (0–3.3)
Env45,493 (63,384–32,652)22.0 (15.8–30.6)
Env peptide 121E25,142 (33,500–18,868)39.8 (29.9–53.0)
FDhNYCBH>3,800,0000.3 (0–3.3)
Env35,347 (48,134–25,957)28.3 (20.8–38.5)
Env peptide 121H39,118 (53,723–28,484)25.6 (18.6–35.1)
FYgNYCBH>3,800,0000.3 (0–3.3)
Nef73,033 (107,962–49,405)13.7 (9.3–20.2)
Nef peptide 3F40,202 (55,348–29,202)24.9 (18.1–34.2)
  • a CTL activity assayed after in vitro SIV-specific stimulation with autologous B-LCL infected with recombinant vaccinia-expressing SIV proteins.

  • b CTL precursor frequency determined by split-well analysis.