Table II.

COX-2 inhibition in vivo reduces PGE2 and IL-10 production by splenic lymphocytesa

Lymphocytes fromIL-10 Production (pg/ml/106 cells)PGE2 Production (pg/ml/106 cells)
Control non-tumor bearer<15*58 ± 23*
Untreated 3LL bearer347.2 ± 12.4515 ± 56
CV-3LL322.6 ± 15.7540 ± 43
4SC7-3LL364.0 ± 22.3583 ± 49
1ASE7-3LL160.0 ± 14.0*220 ± 5*
SC-58236-treated 3LL bearer32.6 ± 4.3*221 ± 67*
Indomethacin-treated 3LL bearer<15*183 ± 21*
  • a On day 14 after tumor inoculation, non-necrotic tumors and splenic lymphocytes were isolated from tumor-bearing mice for determination of PGE2 and IL-10 concentrations. PGE2 and IL-10 levels were evaluated in fresh tumor homogenates and in lymphocyte supernatants after 6 days in culture.

  • b , p < 0.05 compared to untreated tumor-bearing mice.