Table I.

Relationship between processing, transport, and binding features of HLA-B27 ligands and their presence in vivoa

PeptideNameProteasome Yield (%)bTAP PΩ MotifcAffinitydStabilitye DT50 (h)Found in vivo
RRFFPYYV2.5+High13.2 ± 1.8Yes
RRFFPYYVY21.4+High17.9 ± 4.4Yes
SRRFFPYYJ1 0.05 + Low 7.6 ± 0.8 No
SRRFFPYYVJ20.5+High 8.5 ± 1.3 No
SRRFFPYYVYJ34.4+High 9.1 ± 2.2 No
SRRFFPYYVYNJ40.8 Low 9.9 ± 3.3No
RRFFPYYVYNJ52.3High15.2 ± 4.1No
RRFFPYYVYNIJ6 Not found +High17.4 ± 5.1No
  • a Features with the potential to impair the presence of the peptide in the B*2705-bound pool in vivo are underlined.

  • b Peptide yields (see text) are the means of two digestion experiments at 8 h carried out under the same conditions and with similar results.

  • c TAP-binding motifs are from Refs. (14 and 15).

  • d See Fig. 5.

  • e DT50 ±SD. Data are the means of five to seven independent experiments. See text for an assessment of the DT50 values for J2 and J3.