Table III.

Reverse signaling through mTNF leads to down-regulation of LPS-induced release of IL-1 and IL-10a

IL-1IL-10
Control3 (±2)20 (±20)
Anti-TNF3 (±2)17 (±13)
LPS2444 (±677)683 (±70)
Anti-TNF/LPS1968 (±994)460 (±115)
Anti-TNF/2h/LPS836 (±559)b159 (±66)c
  • a The monocytic cell line Mono Mac 6 (see Ref. 10 ) was either left untreated (control), treated with the TNF mAb 195F (anti-TNF), or stimulated with LPS (10 ng/ml) with or without the preincubation with anti-TNF for 30 min and a 2-h delay after anti-TNF stimulation. Supernatants of these preparations were collected 6 h (IL-1) and 24 h (IL-10) after LPS challenge and analyzed for the production of the respective cytokine in an ELISA as described in Materials and Methods. Detection limit was 4 pg/ml, maximal detection was 10,000 pg/ml. Results are given in mean pg/ml (±SD) values of a summary of three experiments.

  • b , p = 0.02 of IL-1 expression after anti-TNF/2h/LPS treatment vs LPS treatment alone.

  • c , p = 0.004 of IL-10 expression after anti-TNF/2h/LPS treatment vs LPS treatment alone.