Reverse signaling through mTNF leads to down-regulation of LPS-induced release of IL-1 and IL-10a
| IL-1 | IL-10 | |
|---|---|---|
| Control | 3 (±2) | 20 (±20) |
| Anti-TNF | 3 (±2) | 17 (±13) |
| LPS | 2444 (±677) | 683 (±70) |
| Anti-TNF/LPS | 1968 (±994) | 460 (±115) |
| Anti-TNF/2h/LPS | 836 (±559)b | 159 (±66)c |
a The monocytic cell line Mono Mac 6 (see Ref. 10 ) was either left untreated (control), treated with the TNF mAb 195F (anti-TNF), or stimulated with LPS (10 ng/ml) with or without the preincubation with anti-TNF for 30 min and a 2-h delay after anti-TNF stimulation. Supernatants of these preparations were collected 6 h (IL-1) and 24 h (IL-10) after LPS challenge and analyzed for the production of the respective cytokine in an ELISA as described in Materials and Methods. Detection limit was 4 pg/ml, maximal detection was 10,000 pg/ml. Results are given in mean pg/ml (±SD) values of a summary of three experiments.
b , p = 0.02 of IL-1 expression after anti-TNF/2h/LPS treatment vs LPS treatment alone.
c , p = 0.004 of IL-10 expression after anti-TNF/2h/LPS treatment vs LPS treatment alone.