Table I.

Metal compounds that inhibit IKK activity in vitroa

Metal CompoundsID50 (μM)
ZnSO48.7
Aurothiomalate10.9
HgCl212.3
Aurothioglucose19.6
AuCl324.1
CuSO426.9
CoCl255.7
Na2Cr2O769.2
  • a RAW 264.7 cells were stimulated with LPS and the cell lysate was immunoprecipitated with anti-IKKα Ab. The IKK immune complex was incubated with GST-IκBα and [γ-32P]ATP in the absence or presence of various concentrations of metal compounds. The reaction mixture was resolved by SDS-PAGE, and phosphorylation of GST-IκBα was measured by phosphor image analysis. The ID50 value was calculated using a Pharm/PCS program (MicroComputer Specialists, Philadelphia, PA). The values are the mean of three determinations.