Table II.

Effect of ENA-78 and GRO-α on the proportion of adherent neutrophils transmigrated through HUVEC treated with 2 or 100 U/ml TNFa

Percentage of Adherent Neutrophils Transmigrated After
3 min5 min9 min14 min
2 U/ml TNF
Activating agent
None3 ± 19 ± 322 ± 730 ± 9
ENA-783 ± 14 ± 14 ± 14 ± 2
GRO-α2 ± 13 ± 15 ± 15 ± 2
100 U/ml TNF
Activating agent
None14 ± 538 ± 1079 ± 884 ± 2
ENA-781 ± 14 ± 213 ± 236 ± 6
GRO-α1 ± 12 ± 112 ± 618 ± 7
  • a For 2 U/ml TNF, agents were perfused over adherent neutrophils. For 100 U/ml TNF, neutrophils were treated with agents immediately before perfusion over the HUVEC. Time is measured from the start of the neutrophil bolus; washout commenced at 4 min. Data are mean ± SEM of three experiments. ANOVA showed a significant inhibitory effect of each activating agent on percentage of migrated neutrophils at 2 or 100 U/ml TNF (p < 0.01).