Table III.

Lymphoid reconstitution of RAG2/γc and RAG2 mice injected with wt and Syk−/− FL cellsa

Spleen (absolute numbers)Intestineb (%)
HostDonorCD4 (106)CD8 (106)γδ T (104)NK T (104)nIEL/ECcγδ Tαβ TICD4CD8βn
RAG2/γc−/−wt11 ± 4.13.1 ± 1.325 ± 1025 ± 14514 ± 749 ± 1412 ± 63.8 ± 3.627 ± 128
RAG2/γc−/− Syk −/− 2.7 ± 0.61.3 ± 0.47 ± 46.5 ± 3715 ± 921 ± 1219 ± 107.5 ± 851 ± 238
RAG2−/−wt8.5 ± 1.13.1 ± 0.529 ± 2.722 ± 0.631042252.5222
RAG2−/− Syk −/− 0.5 ± 0.60.1 ± 0.10.2 ± 0.31.7 ± 0.431.75NDNDND2
  • a Spleens were explanted 8–12 wk after transfer, and cell suspension was enumerated. After calculating the percentage of lymphoid cells (about 65% of total in wt reconstituted spleens and 50% in Syk−/− reconstituted spleens) on the basis of their forward light scatter/side scatter profiles, the numbers of the indicated population were calculated.

  • b IELs were isolated 8–12 wk after transfer. Values are expressed as percentages of IELs out of epithelial cells. All IEL subsets are expressed as percentages of total IELs.

  • c EC, epithelial cells; TI, thymo-independent; ND, not determined.