Table II.

Expression of Syk and ZAP-70 in early thymocytes by in situ hybridizationa

SubsetSykZAP-70
CD44+CD2574 ± 1018 ± 4
CD44+CD25+30 ± 418 ± 5
CD44CD25+22 ± 829 ± 5
CD44CD2540 ± 557 ± 10
ISPb6 ± 337 ± 10
DP4 ± 328 ± 10
SP CD8+4 ± 325c
LN T7 ± 429 ± 8
LN B79 ± 25c
γδ T4 ± 268c
  • a Thymi and LN were explanted from adult B6 mice. The indicated subsets were isolated by FACS sorting and cells were analyzed for Syk and ZAP-70 expression with specific probes. Mean percentages ± SD of positive cells for three independent experiments (unless otherwise stated) are indicated.

  • b ISP, immature single positive.

  • c One experiment only.