Table I.

Thymic cellularity of RAG2/γc and RAG2 mice reconstituted with FL cellsa

HostDonorTotal (106)DPb (106)CD4 SP (106)CD8 SP (106)DN (106)γδTc (104)NK Td (104)n
RAG2/γc−/−wt90 ± 2872 ± 223.9 ± 1.41.2 ± 0.50.5 ± 0.215 ± 1018 ± 65
RAG2/γc−/− Syk −/− 45 ± 2634 ± 192.7 ± 1.50.8 ± 0.40.3 ± 0.14 ± 29.6 ± 27
RAG2−/−wt103 ± 5675 ± 347.9 ± 41.9 ± 0.41.9 ± 0.410 ± 826 ± 173
RAG2−/− Syk −/− 1.3 ± 0.50.3 ± 0.10.2 ± 0.20.3 ± 0.30.3 ± 0.20.07 ± 0.010.6 ± 13
  • a Thymi were explanted 8–12 wk after transfer, and cell suspension was enumerated. After calculating the percentage of lymphoid cells (about 85% of total) on the basis of their forward light scatter/side scatter profiles, the numbers of the indicated population were calculated. Both host-derived and donor-derived cells were included in the calculations.

  • b DP: CD8+CD4+ double-positive thymocytes.

  • c γδT cells were calculated after gating off all cells positive for CD8, CD4, and B220 (>98% of total thymocytes).

  • d NK T cells were calculated after gating off heat-stable antigen-positive immature thymocytes (about 95% of total).