Table III.

Genotype analysis of informative backcross mice with novel NKC STS markers

Locus(BALB/c × C57BL/6J)F1 × BALB/c Mice
Nkrp1a bbbbbbbccbbcbccbcbcbc
Cd69 bbbbbbccbbcbccbcbcbc
Ly49a bbbbbbccbbcbcbbcbcbc
D6Wum15c bbbbbbbbbbbbb
D6Wum13d bbbbbbccbbcbcbbcbcbc
D6Wum9d bbbbbbccbbcbcbbcbcbc
Cmv1 bbbbbbcbbbcbcbbcbcbc
D6Wum16c bbbcbbcbbbcbcbccbbcc
Prp bbbcbbcbbbcbcbccbbcc
(A/J × C57BL/6J)F1 × A/J Mice
Nkrp1 a abbabababba
Ly49a a abbababbbba
D6Wum15c bbbbbbb
D6Wum13 a abbababbbba
D6Wum9d a abbababbbba
Cmv1 a abbabbbbbba
Prp a abbabbbbbba
  • a Nkrp1, Cd69, Ly49a, Cmv1, and Prp alleles in these mice have been reported previously (4 ).

  • b For the recombinant chromosome, b = B6 allele, c = BALB/c allele, and a = A/J allele. For the Cmv1 locus, b = Cmv1r and a and c = Cmv1S.

  • c B6 dominant alleles were determined using radiolabeled NKC locus-specific primers (Table I). D6Wum15-specific primers do not amplify a product from the BALB/c or A/J genomes ((−); and see Table IV). D6Wum16-specific primers amplify a minor product of identical size from the B6 and BALB/c genomes in addition to the expected, major product.

  • d D6Wum13 and D6Wum9 alleles were determined by restriction of backcross mouse DNA and Southern hybridization with the 200H7R and 200H7L Southern probes, respectively (Table I). The 200H7R probe detects 4.8-kb B6 and 5.5-kb BALB/c or A/J HindIII fragments. The 200H7L probe detects 2.5-kb and 3-kb B6 and 3.5-kb BALB/c or A/J HindIII fragments.