Table I.

Predominant usage of Vβ 8.1/8.2 TCR by GAD65-specific T cellsa

T Cell HybridomaTCR Vβ ExpressionThymidine Uptake
No AgGAD65
NOD-IL-4
1F128.1, 8.23,76820,092
1B58.1, 8.232,08962,198
3E58.1, 8.220,82272,054
1D68.1, 8.22,62445,908
3B48.1, 8.23,728107,055
3D11422,44756,550
2B45.1, 5.211,33960,023
NOD
6G28.1, 8.2931107,261
7F78.1, 8.25,31332,816
7C48.1, 8.23,75425,545
8B98.1, 8.21,63836,834
7D18.310,53756,361
6G58.31,11087,419
6H710b2,86533,742
8B847,920117,082
  • a IL-2 secretion by T cell hybridomas upon 24-h incubation with GAD65. The TCR Vβ expression by the respective hybridoma was determined by FACS.