Table III.

Activity of IL-8 transcriptional reporter deletion mutants in HT-29 cells infected with enteroinvasive bacteria or stimulated with TNF-αa

IL-8-CAT Construct (bp)S. dublin InfectedY. enterocolitica InfectedTNF-α Stimulated
−14814.9 ± 1.8b7.1 ± 2.19.0 ± 3.0
−5463.4 ± 1.04.9 ± 1.35.5 ± 1.4
−2723.4 ± 0.73.4 ± 0.84.8 ± 1.8
−988.6 ± 1.510.7 ± 1.914.3 ± 5.5
−80.6 ± 0.10.9 ± 0.20.6 ± 0.1
  • a HT-29 cells were transiently transfected with the various 5′-deletion mutant IL-8-CAT transcriptional reporters, as indicated, and infected with S. dublin or Y. enterocolitica or stimulated with TNF-α (20 ng/ml) as described in Materials and Methods.

  • b CAT activity is given as fold increase over unstimulated cells. Data are means ± SEM of three or more experiments.