Table I.

Oligonucleotides of the ISRE regions of the various HLA class I loci used for EMSAsa

ISRE-AcggatccctccgcAGTTTCTTTTCTccgagct
ISRE-BEcggatccactcccacgAGTTTCACTTCTtctgagct
ISRE-B/CcggatccactcccctgAGTTTCACTTCTtctgagct
HLA-EcggatcctctgcAGTTTCCCGTTCctgagct
HLA-FcggatcctctccccagAGTTTCTCTTTCtctgagct
HLA-GcggatccATTCTCTCCTCCttctcctgagct
ConsensusAGTTTCNNTTTC
  • a The putative ISRE binding motif sequence is shown in capitals (16). The ISREs of most HLA-B loci (ISRE-BE) contain a putative E box (underlined), except the ISRE of HLA-B7, HLA-B8, HLA-B38, HLA-B39, HLA-B42, HLA-B64, and HLA-B65. The ISRE probes of the latter loci are therefore grouped with the ISREs of the HLA-C loci (ISRE-B/C). The consensus ISRE is given as reference (5). CG-rich sequences (putative Sp1 binding sites) are present in the ISRE and flanking sequences of HLA-A, HLA-B, HLA-C, HLA-F, and HLA-G. The sequences are flanked 5′ by KpnI and BamHI sites and 3′ by a SacI site. The corresponding complementary strands are not shown.