Table I.

IFN-γ, IL-10, and MCP-1 production in cultures containing BMNK cells

SampleaIFN-γ (ng/ml)bIL-10 (pg/ml)bMCP-1 (pg/ml)b
Expt. 1
T, APC, Con A3563984
+BM (1:1)c4111381
BM+ Con ANDdND29
Expt. 2
T, APC, fraction 1 (1:8)eNDND94
T, APC, fraction 2 (1:8)eNDND11
T, APC, Con A8173524
+Fraction 1 (1:8)NTf328411
+Fraction 2 (1:8)NT48337
+Fraction 1 (1:16)57373237
+Fraction 2 (1:16)9363234
+Fraction 1 (1:32)64432122
+Fraction 2 (1:32)8465230
Expt. 3
T, APC, fraction 1 (1:8)eNDND54
T, APC, fraction 2 (1:8)eNDND22
T, APC, MBP6.67625
+Fraction 1 (1:8)3.132254
+Fraction 2 (1:8)5.47338
+Fraction 1 (1:16)3.337152
+Fraction 2 (1:16)5.56738
+Fraction 1 (1:32)4.14778
+Fraction 2 (1:32)5.75233
  • a T cells were stimulated for 72 h with Con A (expts. 1 and 2) or MBP (expt. 3) and APCs, with/without BM cells. Supernatants were tested by ELISA.

  • b SDs were <10% for all samples.

  • c BM cells were not fractionated in this experiment.

  • d ND, not detected (OD < control without cytokine reference standard).

  • e Background values (no Con A or MBP).

  • f NT, not tested.