Table I.

Similar proliferative response of DN cells from H-2b 2C and H-2b β2m−/− 2C mice to the p2Ca/Ld liganda

p2Ca (μM)H-2b 2C DNH-2b β2m−/− 2C DN
01,350 ± 4541,005 ± 326
0.0012,496 ± 8453,675 ± 1,050
0.019,139 ± 1,76115,155 ± 820
0.150,647 ± 2,85430,334 ± 1,895
1.0101,742 ± 6,737109,081 ± 2,283
10122,204 ± 1,771101,845 ± 4,031
  • a Each culture well contained 1 × 104 purified CD4CD81B2+ lymph node cells from either H-2b 2C or H-2b β2m−/− 2C mice and 5 × 104 mitomycin-treated T2-Ld cells and the indicated concentration of the p2Ca peptide. The cultures were set up in triplicates and assayed for [3H]thymidine incorporation in the last 8 h of a 72-h culture period. The results of the proliferation assay are expressed as mean ± SD. Similar results were obtained from two separate experiments.