Table II.

Characteristics of clones derived from bulk transduced PBL cultures

CulturePhenotypeaTotal No. of ClonesIFN-γ Release vs. T2 + m9-27bIFN-γ Release vs. TumorcSpecific Lysis of T2 + m9-27dSpecific Lysis of 888-A2e
PBL-65CD444000
CD888122
CD4/CD811110
PBL-66CD40
CD899998
PBL-67CD40
CD812128125
  • a Cell-surface immunofluorescence analysis. CD4/CD8: cloid containing both CD4+ and CD8+ T cells.

  • b Specific IFN-γ release measured in culture supernatants from clones cocultured with m9-27 peptide-pulsed T2 cells.

  • c Specific IFN-γ release measured in culture supernatants from clones cocultured with HLA-A2+ melanoma cell lines.

  • d,e Specific lysis measured in 4-h 51Cr release assays at an E:T ratio of 80:1. Clones that were considered to be specific exhibited at least 15% lysis over background (T2 + m9-27, range 18–100%, mean 63.5%; 888-A2+ MEL, range 15–85%, mean 42.1%). In all experiments, background lysis of T2 + g9-209 or 888 MEL was <10%.