Characteristics of clones derived from bulk transduced PBL cultures
| Culture | Phenotypea | Total No. of Clones | IFN-γ Release vs. T2 + m9-27b | IFN-γ Release vs. Tumorc | Specific Lysis of T2 + m9-27d | Specific Lysis of 888-A2e |
|---|---|---|---|---|---|---|
| PBL-65 | CD4 | 4 | 4 | 0 | 0 | 0 |
| CD8 | 8 | 8 | 1 | 2 | 2 | |
| CD4/CD8 | 1 | 1 | 1 | 1 | 0 | |
| PBL-66 | CD4 | 0 | – | – | – | – |
| CD8 | 9 | 9 | 9 | 9 | 8 | |
| PBL-67 | CD4 | 0 | – | – | – | – |
| CD8 | 12 | 12 | 8 | 12 | 5 |
a Cell-surface immunofluorescence analysis. CD4/CD8: cloid containing both CD4+ and CD8+ T cells.
b Specific IFN-γ release measured in culture supernatants from clones cocultured with m9-27 peptide-pulsed T2 cells.
c Specific IFN-γ release measured in culture supernatants from clones cocultured with HLA-A2+ melanoma cell lines.
d,e Specific lysis measured in 4-h 51Cr release assays at an E:T ratio of 80:1. Clones that were considered to be specific exhibited at least 15% lysis over background (T2 + m9-27, range 18–100%, mean 63.5%; 888-A2+ MEL, range 15–85%, mean 42.1%). In all experiments, background lysis of T2 + g9-209 or 888 MEL was <10%.