Table I.

MART-1 Ag recognition by TIL 5 TCR transduced bulk PBL cultures

StimulatorsHLA-A2Responders (pg/ml IFN-γ released)
Medium65 SHAMa65 TRANa1235 TILb1520 TILb
Medium0.060.716.163.129.5
T2 alone+0.762.965.851.8140.9
T2+ g9-209+0.470.180.945.4 12875.0 c
T2+ m9-27+0.654.6 980.0 8082.5 158.1
397 MEL0.067.471.142.021.6
397 A2 MEL+1.674.8129.8 2210.0 1717.5
624-28 MEL0.358.2131.136.615.6
624-38 MEL+12.659.0205.0 5612.5 3515.0
1300 MEL+0.450.2243.6 11902.5 13627.5
StimulatorsHLA-A2Medium66 SHAM66 TRAN67 SHAM67 TRAN1235 TIL1520 TIL
Medium1.58.213.28.314.739.572.5
T2 alone+1.615.715.715.516.516.4109.6
T2+ g9-209+0.413.315.216.712.232.6 >2000
T2+ m9-27+1.615.3 506.5 13.2 214.3 >2000 119.3
397 MEL1.115.356.819.546.134.819.9
397 A2 MEL+1.511.774.613.851.0 >2000 >2000
1011 MEL1.111.717.79.915.034.9170.8
888 A2 MEL+1.239.3 603.1 31.4 473.9 >2000 >2000
1558 MEL+0.891.9 292.0 57.6 174.0 226.5 252.0
1300 MEL+0.056.8 249.1 51.0 163.1 >2000 >2000
SK23 MEL+1.620.9 281.4 22.1 177.9 >2000 >2000
  • a Abbreviations: SHAM, untransduced; TRAN, transduced.

  • b 1235 TIL is a MART-1-reactive culture that recognizes the m9-27 peptide. 1520 TIL is a gp100-reactive culture and recognizes the g9-209 peptide.

  • c Bold results represent specific IFN-γ release that is >50 pg/ml and at least 2-fold over background secretion. Values marked >2000 were in excess of the maximum standard used in that assay. Results presented are representative of multiple experiments.