Test Agsa
Ag | Description/Source | Derivation, Comments |
---|---|---|
PI | Lilly Pharm. | E. coli, full-length* |
IA-2 | Bayer Pharm. | E. coli, “ICA512”* |
GAD65 | Peptide 524 | SRLSKVAPVIKARMMEYGTT* |
HSP60 | Peptide 277 | VLGGGCALLRCIPALDSLTPANED* |
ICA69 | β-isoform | E. coli* |
α-isoform | Baculovirus† | |
ICA69-36 “Tep69” | AFIKATGKKEDE, homolog BSA-148* | |
ICA69-202 | KNFDKLKMDVCQ, homolog BSA-394† | |
ICA69-350 | SEEGACLGPVAG, homolog BSA-193† | |
BSA | Sigma | Fraction V* |
BSA-148 “ABBOS” | FKADEKKFWGKYL, homolog ICA69-36* | |
BSA-394 | TSVFDKLKHLVD, homolog ICA69-202† | |
BSA-193 | EDKGACLLPKIE, homolog ICA69-350† | |
Tetanus toxoid | PMC Canada | Pharmaceutical grade* |
OVA | Sigma | Fraction V* |
OVA152 | EYQDNRVSFLGHFI* | |
Human hemoglob. | Sigma | Purified† |
Human cytochr. c | Sigma | Purified† |
Human actin | Sigma | Purified† |
a Description of the test antigens/peptides used, not all could be tested in all study subjects. Eleven Ags (marked with an asterisk) were used routinely; the remainder (marked with a dagger) were tested only in subsets of probands to answer specific questions. Peptide names indicate origin and N-terminal amino acid position. Residues printed in bold identify homologies described in the text.