Table I.

Quantitative RT-PCR primers and probes

IFN-γ(+)-AGCTCTGCATCGTTTTGGGTTa
(−)-GTTCCATTATCCGCTACATCTGAA
FAM-TCTTGGCTGTTACTGCCAGGACCCA-TAMRA
GM-CSF(+)-CCCGCCTGGAGCTGTACA
(−)-AATCTGGGTTGCACAGGAAGTTT
FAM CCCTTGACCATGATGGCCAGCC-TAMRA
TNF-α(+)-CCCCAGGGACCTCTCTCTAATC
(−)-TACAACATGGGCTACAGGCTTG
FAM-CAGTCAGATCATCTTCTCGAACCCCGAG-TAMRA
IL-2(+)-ACCAGGATGCTCACATTTAAGTTTT
(−)-GAGGTTTGAGTTCTTCTTCTAGACACTG
FAM-CATGCCCAAGAAGGCCACAGAACTG-TAMRA
CD25(+)-AGCCAGTGGACCAAGCGA
(−)-TGTGGCTTCATTTTCCCATG
FAM-TCCAGGTCACTGCAGGGAACCTCC-TAMRA
CD69(+)-AACAGCTCTTTGCATCCGGA
(−)-ACCCTTCATGACGTGTTGAGAA
FAM-CAAGAAAATGATGCCACCAGTCCCCA-TAMRA
CD8(+)-CCCTGAGCAACTCCATCATGT
(−)-GTGGGCTTCGCTGGCA
FAM-TCAGCCACTTCGTGCCGGTCTTC-TAMRA
CD4(+)-GTCCCTTTTAGGCACTTGCTTCT
(−)-TCTTTCCCTGAGTGGCTGCT
FAM-TGCTGCAACTGGCGCTCCTCC-TAMRA
gp100(+)-GGTTCCTTTTCCGTCACCCT
(−)-CTCACCGGACGGCACAG
FAM-ACATTGTCCAGGGTATTGAAAGTGCCGAGAT-TAMRA
β-Actin(+)-GGCACCCAGCACAATGAAG
(−)-GCCGATCCACACGGAGTACT
FAM-TCAAGATCATTGCTCCTCCTGAGCGC-TAMRA
  • a (+), forward primers: (−), reverse primers; FAM-TAMRA, probe. Concentrations used: 400 nM primers, 150 nM probe.