Table IV.

Calculated precursor frequenciesa of Flu-specific CD8+ cells on day 10 (peptide-specific/10e6 CD8+ cells)

d1Flu Stimulationb
PrePostPrePost
Peptide alonecP13572,2547,2806,538
P22,1773,06419,34733,450
P31,1151,2744971,334
P42,8701d43,22972,034
P54,7325,70317,34818,676
P66,8207,901173,757131,309
P74152,38930,49232,211
Peptide+ IL-12P82,0021,23746,27527,266
P948066712,15012,462
P108361,38643613,785
P112,0544,16415,34330,713
P1221,58316,650228,989121,649
Peptide+ IL-2P131,99218,1898,288
P146631,02663,54621,965
P151,5194,94143,60778,601
P162,2281,47334,7092,864
P1714,4966,30975,45599,607
P1842111,6122,285
P191,6901,65638,73253,972
P209082,68720,01153,972
P2183826065,978115,133
P221,8381,3417,0717,373
P231,3744652614,267
  • a Frequency calculation: [URQ/(LRQ + URQ) × 10e6]epitope-specific − [URQ/(LRQ + URQ) × 10e6]background.

  • b Ten-day in vitro cultures stimulated with Flu.

  • c Treatment group.

  • d Calculated frequencies below or equal to zero were arbitrarily set to 1 to permit statistical analysis involving log10. No increase in g209/g209-2M-specific TCPF (data not shown).