Table III.

Calculated precursor frequenciesa of g209-2M-specific CD8+ cells on day 10 (peptide-specific/10e6 CD8+ cells)

d1g209 Stimulationbg209-2M Stimulation
PrePostPrePostPrePost
Peptide alonecP16389,6644277,20398611,271
P22,2184,9167497981,5824,576
P31,5584,301674129,367854113,703
P42,58723,952283249,081280296,596
P51,4163,44053715,1612,78060,936
P65,47312,7233,842248,9731,000287,991
P74081,5642893,14031813,412
Peptide+ IL-12P85005,6091d137,7401,202226,460
P94011,7144224,9351,08543,216
P102433,4885403,29171019,966
P1112,49013,6181,31858,34569672,815
P122,27424,5761287,9542,058291,347
Peptide+ IL-2P131,73585322,51725,93026,03719,794
P141,6072,3353,8494,8607,2212,924
P151,79110,0438735,31356311,763
P164,6202,7718722,8771,8633,066
P173005333651,2272701,502
P181,75527218,27223,6777,40415,465
P191,0512517533,4663553,379
P207699792,8553,4661,3853,379
P2189530655616,69968641,414
P224,5071,338754935349715
P232,4268695423,73826010,406
  • a Frequency calculation: [URQ/(LRQ + URQ) × 10e6]epitope-specific − [URQ/(LRQ + URQ) × 10e6]background.

  • b Ten-day in vitro cultures stimulated with g209 or g209-2M.

  • c Treatment group.

  • d Calculated frequencies below or equal to zero were arbitrarily set to 1 to permit statistical analysis involving log10. Control cultures stimulated with Flu or without any peptide did not demonstrate an increase in g209/g209-2M-specific TCPF (data not shown).