Table II.

Calculated precursor frequenciesa of g209-specific CD8+ cells on day 10 (peptide-specific/10e6 CD8+ cells)

d1g209 Stimulationbg209-2M Stimulation
PrePostPrePostPrePost
Peptide alonecP13562,10953818,29142920,655
P26672,8632,8952,3231,2112,595
P32,0833,451460133,69858598,962
P41,85414,806189196,025379197,705
P51044,40254012,2061,56916,637
P64,7799,1374,036240,755741231,598
P74541,9881759713703,779
Peptide+ IL-12P85084,1771d107,871613123,606
P94972,1996594,0181,01542,937
P102823,1352681,0785866,242
P118,5666,8341,32147,49027973,518
P124,77014,5491184,1581,397146,721
Peptide+ IL-2P132,14112116,14025,14116,30413,802
P141,8951,7143,5192,5874,3703,538
P151,8429,5241,0734,2032339,625
P167,2254,8872,2833,4171,6541,282
P1714336289875961,123
P182,34597116,38019,7026,42511,965
P1961627112,949129725
P202,5589503,6632,0391,388725
P211,8702395647,45418214,645
P223,1341,8131,1421,327172508
P232,4271,0571242,12913,023
  • a Frequency calculation: [URQ/(LRQ + URQ) × 10e6]epitope-specific − [URQ/(LRQ + URQ) × 10e6]background.

  • b Ten-day in vitro cultures stimulated with g209 or g209-2M.

  • c Treatment group.

  • d Calculated frequencies below or equal to zero were arbitrarily set to 1 to permit statistical analysis involving log10. Control cultures stimulated with Flu or without any peptide did not demonstrate an increase in g209/g209-2M-specific TCPF (data not shown).