Table I.

Calculated precursor frequenciesa of peptide-specific CD8+ cells in PBMC on day 1 (peptide-specific/10e6 CD8+ cells)

g209bg209-2MFluDaysc
PrePostPrePostPrePost
Peptide alonedP13562,1096389,6643572,25441
P26672,8632,2184,9162,1773,06441
P32,0833,4511,5584,3011,1151,274223
P41,85414,8062,58723,9522,8701e43
P51044,4021,4163,4404,7325,70346
P64,7799,1375,47312,7236,8207,90194
P74541,9884081,5644152,38941
Peptide+ IL-12P85084,1775005,6092,0021,23742
P94972,1994011,71448066790
P102823,1352433,4888361,38640
P118,5666,83412,49013,6182,0544,16442
P124,77014,5492,27424,57621,58316,65042
Peptide+ IL-2P132,1411211,7358531,9921283
P141,8951,7141,6072,3356631,026199
P151,8429,5241,79110,0431,5194,94189
P167,2254,8874,6202,7712,2281,473184
P17143330053314,4966,30945
P182,3459711,7552724211145
P196162711,0512511,6901,65654
P202,5589507699799082,68750
P211,87023989530683826094
P223,1341,8134,5071,3381,8381,34141
P232,4271,0572,4268691,37446585
  • a Frequency calculation: [URQ/(LRQ + URQ) × 10e6]epitope-specific − [URQ/(LRQ + URQ) × 10e6]background.

  • b Staining with g209-, g209-2M-, or Flu-tHLA.

  • c Time interval between the pre- and postvaccination sample.

  • d Treatment group.

  • e Calculated frequencies below or equal to zero were arbitrarily set to 1 to permit statistical analysis involving log10.