Table I.

Calculated precursor frequencies^a of peptide-specific CD8⁺ cells in PBMC on day 1 (peptide-specific/10e6 CD8⁺ cells)

		g209^b		g209-2M		Flu		Days^c
		Pre	Post	Pre	Post	Pre	Post	Days^c
Peptide alone^d	P1	356	2,109	638	9,664	357	2,254	41
	P2	667	2,863	2,218	4,916	2,177	3,064	41
	P3	2,083	3,451	1,558	4,301	1,115	1,274	223
	P4	1,854	14,806	2,587	23,952	2,870	1^e	43
	P5	104	4,402	1,416	3,440	4,732	5,703	46
	P6	4,779	9,137	5,473	12,723	6,820	7,901	94
	P7	454	1,988	408	1,564	415	2,389	41

Peptide+ IL-12	P8	508	4,177	500	5,609	2,002	1,237	42
	P9	497	2,199	401	1,714	480	667	90
	P10	282	3,135	243	3,488	836	1,386	40
	P11	8,566	6,834	12,490	13,618	2,054	4,164	42
	P12	4,770	14,549	2,274	24,576	21,583	16,650	42

Peptide+ IL-2	P13	2,141	121	1,735	853	1,992	1	283
	P14	1,895	1,714	1,607	2,335	663	1,026	199
	P15	1,842	9,524	1,791	10,043	1,519	4,941	89
	P16	7,225	4,887	4,620	2,771	2,228	1,473	184
	P17	1	433	300	533	14,496	6,309	45
	P18	2,345	971	1,755	272	421	1	145
	P19	616	271	1,051	251	1,690	1,656	54
	P20	2,558	950	769	979	908	2,687	50
	P21	1,870	239	895	306	838	260	94
	P22	3,134	1,813	4,507	1,338	1,838	1,341	41
	P23	2,427	1,057	2,426	869	1,374	465	85

a Frequency calculation: [URQ/(LRQ + URQ) × 10e6]_{epitope-specific} − [URQ/(LRQ + URQ) × 10e6]_background.
b Staining with g209-, g209-2M-, or Flu-tHLA.
c Time interval between the pre- and postvaccination sample.
d Treatment group.
e Calculated frequencies below or equal to zero were arbitrarily set to 1 to permit statistical analysis involving log₁₀.