Table I.

Effect of apoptotic cell phagocytosis on chemokine and TNF release by macrophagesa

CytokineEffect of Apoptotic Cell Uptake (% control)
In unstimulated J774 cellsIn LPS-stimulated J774 cells
TNF-α63 ± 3 (n = 4)*41 ± 5 (n = 11)**
Mip-261 ± 11 (n = 4)*60 ± 3 (n = 11)**
KC35 ± 14 (n = 4)*50 ± 5 (n = 11)**
Mip-1α51 ± 9 (n = 4)*61 ± 4 (n = 10)**
MCP-1/JE102 ± 4 (n = 4)95 ± 7 (n = 9)
IL-1080 ± 9 (n = 6)*96 ± 4 (n = 11)
  • a Mouse J774 cells were cultured for 90 min in the absence or presence of apoptotic Jurkat cells (at a 1:5 ratio). Uningested Jurkat cells were removed by mild washings prior to further culture for 12 h with or without 1 ng/ml LPS. Culture supernatants were analyzed by ELISA. Values are mean ± SEM of the indicated number of experiments.

  • b , Significantly different than unstimulated J774 cells at p ≤ 0.04.

  • c ∗, Significantly different than LPS-treated J774 cells at p ≤ 0.0001.