Table II.

PS-, but neither PE- nor PS/met-, ODNs stimulate human B cell proliferationa

Reagent Added in Bulk Culture (10 μg/ml)B Cell Proliferation (MI)b
Expt. 1Expt. 2
PEPSPS/metPEPSPS/met
DSP301.110.02.10.927.04.0
DSP171.313.02.00.721.03.5
3Da0.96.51.81.127.84.2
21051.06.03.01.117.05.0
Myco0.810.42.31.325.07.5
13261.07.01.40.922.54.0
Poly CG1.11.21.00.71.10.9
  • a Highly purified B cells from two healthy volunteers were stimulated for 2 days in the presence or absence of PE-, PS-, or PS/met-ODNs (10 μg/ml). After 16 h pulsing with [3H]TdR, cultures were harvested and radionuclide uptake measured by scintillation counting, as described in Materials and Methods.

  • b MI was calculated as the ratio between the mean values of cpm obtained in ODN-stimulated cultures and cpm obtained in the presence of medium alone.