Table I.

COX activity in Jurkat cells and T lymphocytes after PMA plus Ion or anti-CD3 + anti-CD28 treatment

Cell TypeTreatmentaPGs (ng/107 cells)b
Jurkat cellsControl0.13 ± 0.02c
PMA+ Ion2.44 ± 0.18
T lymphocytesControl0.32 ± 0.01
Anti-CD3+ anti-CD288.88 ± 1.52
  • a Cells were incubated for 24 h with PMA + Ion or anti-CD3 + anti-CD28 Abs as described in Materials and Methods.

  • b PG production in cell sonicates after 15-min incubation with AA measured by an standard EIA assay as described in Materials and Methods.

  • c Mean ± SD.