COX activity in Jurkat cells and T lymphocytes after PMA plus Ion or anti-CD3 + anti-CD28 treatment
| Cell Type | Treatmenta | PGs (ng/107 cells)b |
|---|---|---|
| Jurkat cells | Control | 0.13 ± 0.02c |
| PMA+ Ion | 2.44 ± 0.18 | |
| T lymphocytes | Control | 0.32 ± 0.01 |
| Anti-CD3+ anti-CD28 | 8.88 ± 1.52 |
a Cells were incubated for 24 h with PMA + Ion or anti-CD3 + anti-CD28 Abs as described in Materials and Methods.
b PG production in cell sonicates after 15-min incubation with AA measured by an standard EIA assay as described in Materials and Methods.
c Mean ± SD.