Table III.

Inhibition of G0 IgG uptake by dendritic cells in the presence of mannan

IgG Concentration (μg/ml)Aggregated G2a% InhibitionAggregated G0% Inhibition
04bNA4NA
527<01344
1040<02365
20106193972
40168206478
60200359778
  • a IgG was aggregated by cross-linking with Cy3 bisfunctional dye (Amersham, Pittsburgh, PA), which was also used as the fluorochrome for detection of ligand uptake. SDS-PAGE was used to confirm cross-linking of the protein. Dendritic cells were incubated with the indicated ligands in the presence of 100 μg/ml mannan, as described in Fig. 5.

  • b Mean fluorescence intensity.