Table I.

Serum TNF and IFN-γ levels in mice deficient in different lymphocyte subsetsa

Mouse PhenotypeTreatmentng/ml (± SEM)
TNFIFN-γ
ImmunocompetentLPS0.7 ± 0.3≤0.6
LCMV + LPS27.9 ± 8.7b42.4 ± 19.0b
SCID (NK+TB)LPS0.8 ± 0.3≤0.6
LCMV + LPS23.0 ± 5.5b ,c3.2 ± 1.7b ,e
RAG-1−/− (NK+TB)LPS0.9 ± 0.80.8 ± 0.5
LCMV + LPS31.2 ± 4.6b ,c1.6 ± 0.4e
E26 (NKT)LPS2.5 ± 0.2≤0.6
LCMV + LPS11.6 ± 3.2c ,d≤0.6
  • a Values are the result of several combined experiments and are represented as mean ± SEM, with 3 to 6 mice per group. Where ELISA measurements were below the limit of detection, means are expressed as greater than or equal to the value of the limit of detection. Immunocompetent mice were C57BL/6, T and B cell-deficient animals were C57BL/6-SCID and C57BL/6-RAG-1−/− mice, and NK and T cell-deficient animals were CBA × B6 transgenic E26 mice.

  • b p ≤ 0.01 vs LPS treatment in the same mouse strain.

  • c NS vs LCMV + LPS treatment in C57BL/6 mice.

  • d p = 0.07 vs LPS treatment in the same mouse strain.

  • e p ≤ 0.01 vs LCMV + LPS treatment in C57BL/6 mice.