Table I.

Pattern of reactivity of hybrids derived from B6-stimulated K14 splenocytesa

Hybrid No.TCR UsageMHC Reactivity
VαVβB6BALB/cAKR
114+
2+
314+
46++
5118++
65++
75+++
85++
913+
1038+
116++
128++
138+++
1415+++
1512+++
163+±
17215+
18+
1928++
208++
212+
222+
232+++
245+++
258++
263+
27+
2814+
296++
3012+++
31+
3214+
332+
3414+
356++
36+++
37+++
383+
396++
402+
  • a Hybrids were stimulated with splenocytes from the indicated mice, and IL-2 in the supernatant was assayed using the HT-2 cell line. A positive score indicates IL-2 production at least 5-fold greater than background. Expression of TCR Vα 2, 3, 8, or 11 and Vβ 2, 3, 5, 6, 8, 9, 10, 11, 12, 13, or 14 was determined by flow cytometry. Blanks indicate hybrids reactive with none of the tested Abs. The expression of Vβ 15 by hybrid number 17 was determined by PCR.