Table III.

Correlation between severity of thyroiditis and orbital pathology in BALB/c mice

TreatmentaThyroiditisb% InterstitiumcB:T Cell RatiodOrbital Pathologye% Inter- stitiumc
120.87.6
Examined 8 wk post receipt MBP-ECD primed whole T cells130.97.3
+201.28.8
+201.39.5
+191.38.9
+241.7+14.2
+251.8+14.4
+281.8+16.6
+291.9+15.3
+281.9+17.5
Examined 8 wk post receipt MBP-ECD primed CD4+ cells150.88.7
+191.29.5
+201.28.4
+281.8+18
+301.9+17.7
Examined 12 wk post receipt MBP-ECD primed whole T cells+261.6+17.2
+261.7+16.6
+281.7+18.6
+281.8+16.4
+301.9+18.7
  • a Treatments are as follows: 8 wk whole T, mice examined 8 wk after transfer of T cells primed with the extracellular domain of the TSHR fusion protein and not fractionated; 8 wk CD4+, mice examined 8 wk after transfer of T cells primed with the extracellular domain of the TSHR fusion protein and fractionated to yield CD4+ enriched; 12 wk whole T, mice examined 12 wk after transfer of T cells primed with the extracellular domain of the TSHR fusion protein and not fractionated.

  • b +, presence of thyroiditis; −, absence of thyroiditis.

  • c The percent interstitium is estimated on toluidine blue-stained sections by point counting.

  • d The numbers of intrathyroidal CD3+ T cells and B cells in BALB/c mice were evaluated at a magnification of ×250 in 10 microscopic fields chosen at random and given as the B:T cell ratio.

  • e +, presence of orbital pathology; −, absence of orbital pathology.