Table IV.

Removal of IL-6, TGF-β, and CGRP by immunoprecipitation had no effect on the ability of GCS to alter IL-12 and IL-10 cytokine profiles of PBMC

PBMCCulture Without or With Immunoprecipitated GCSa (final 1:40)Cytokine Determination by ELISA (pg/ml)
IL-12 (p40)IL-10IFN-γ
Unstimulated050
SAC337213391120
SACGCS (none Ab)1263310478
SACGCS (anti-IL-6)1283292073
SACGCS (anti-TGF-β)10923072137
SACGCS (anti-CGRP)1075301798
SACGCS (anti-IL-6 and TGF-β)12562678225
SACGCS (anti-IL-6, TGF-β, and CGRP)12962496209
  • a The experimental procedure is shown in Materials and Methods.