Table II.

Effect of incubation of PBMC with GCS for different time intervals on SAC-stimulated IL-12, IL-10, and IFN-γ production

PBMC Were Preincubated in 37°C for 1 hContent of 24 h CulturedCytokine Determination by ELISA (pg/ml)
IL-12 (p70)IL-12 (p40)IL-10IFN-γ
PBMCSAC34.3 ± 8.64870 ± 476932 ± 1893327 ± 719
PBMCSAC+GCSa > 10a↓3.4 ± 0.3b2.2 ± 0.2↓4.2 ± 0.5
Preincubation with GCS for 1 hSAC↓ > 10a↓3.6 ± 0.2↑3.5 ± 0.6↓5.9 ± 1.1
Preincubation with GCS for 15–30 minSAC↓ > 10a↓3.4 ± 0.3↑3.0 ± 0.4↓3.3 ± 0.5
Preincubation with GCS for 3–10 minSAC↓6.3 ± 1.1↓1.8 ± 0.1↑2.5 ± 0.2↓2.8 ± 0.4
  • a,b The fold increase (↑) or fold decrease (↓) change from SAC-stimulated PBMC based on the mean of four independent experiments (SEM).

  • a Below detectable level. GCS was used at final dilution of 1/20 in the culture and preincubation system.