Table II.

Primer sequences for multiplex PCR

PrimerSequencePositionReaction Seta
Vβ15′-CAA CAG TTC CCT GAC TTG CAC-3′84A
Vβ25′-TCA ACC ATG CAA GCC TGA CCT-3′86B
Vβ35′-TCT AGA GAG AAG AAG GAG CGC-3′86C
Vβ45′-CAT ATG AGA GTG GAT TTG TCA TT-3′122B
Vβ5S15′-TTC AGT GAG ACA CAG AGA AAC-3′135D
Vβ5S25′-CCT AAC TAT AGC TCT GAG CTG-3′75D
Vβ65′-AGG CCT GAG GGA TCC GTC TC-3′81E
Vβ75′-CTG AAT GCC CCA ACA GCT CTC-3′86F
Vβ85′-TAC TTT AAC AAC AAC GTT CCG-3′144B
Vβ95′-AAA TCT CCA GAC AAA GCT CAC-3′84G
Vβ105′-CAA AAA CTC ATC CTG TAC CTT-3′76L
Vβ115′-ACA GTC TCC AGA ATA AGG ACG-3′90H
Vβ125′-GAC AAA GGA GAA GTC TCA GAT-3′117H
Vβ13S15′-GAC CAA GGA GAA GTC CCC AAT-3′117C
Vβ13S25′-GTT GGT GAG GGT ACA ACT GCC-3′135I
Vβ145′-TCT CGA AAA GAG AAG AGG AAT-3′84J
Vβ155′-GTC TCT CGA CAG GCA CAG GCT-3′87I
Vβ165′-GAG TCT AAA CAG GAT GAG TCC-3′132G
Vβ175′-CAC AGA TAG TAA ATG ACT TTC AG-3′137J
Vβ185′-GAG TCA GGA ATG CCA AAG GAA-3′117A
Vβ195′-CCC CAA GAA CGC ACC CTG C-3′79K
Vβ205′-TCT GAG GTG CCC CAG AAT CTC-3′111E
Vβ215′-GAT ATG AGA ATG AGG AAG CAG-3′143L
Vβ225′-CAG AGA AGT CTG AAA TAT TCG A-3′122F
Vβ235′-TCA TTT CGT TTT ATG AAA AGA TGC-3′146A
Vβ245′-AAA GAT TTT AAC AAT GAA GCA GAC-3′129K
Cβ145′-CTC AGC TCC ACG TG-3′
CβR5′-CTT CTG ATG GCT CAA ACA C-3′
  • a A total of 12 reaction sets (A—L) with two or more primers were used for multiplex PCR, as described in Materials and Methods.