Table II.

Fractionation of APCs from lymphoid organs during acute Sendai virus infection

Cell FractionaUnsortedCD45CD11bCD11cCD45R+CD11b+CD45RCD11c+CD45R\E
MLNb c \E
5204 H56 × 1043.1 × 104 (1.94d )ND2.8 × 103 (21.4)2.3 × 102 (261)\E
1.25 × 105ND>1.25 × 106 (<0.1)5.75 × 103 (21.7)8.13 × 103 (15.3)\E
5205 G96 × 1043.25 × 105 (0.2)ND4.25 × 103 (14.1)1.55 × 103 (38.7)\E
5 × 105ND>1.25 × 106 (<0.1)>1.15 × 104 (<43)8.13 × 103 (61.5)\E
Spleen\E
5204 H5106>106ND6 × 105 (1.7)1.25 × 105 (4)\E
>106ND>1.25 × 106 (<0.8)>1.5 × 105 (<6.7)4.5 × 105 (>2.2)\E
5205 G9>106>106ND>6 × 105>2.5 × 105\E
>106ND>1.25 × 106>1.25 × 105>9 × 105
  • a Cells from MLNs or spleen were stained with anti-CD45R (B220) Cy5, anti-CD11b PE and, anti-CD11c FITC Abs and the following populations were collected by FACS: CD45R+ cells (B cells), CD11b+CD45R (macrophages), and CD11c+CD45R cells (DCs).

  • b Reciprocal cell frequencies are presented. Where reciprocal frequencies are expressed as greater than a certain value, this means that no significant response was detected from the lacZ-inducible hybrids at the maximum number of cells per well of this APC type. The cell frequency was calculated from the minimum number of APCs that produced at least twice the number of lacZ+ cells that were observed in control wells (those containing APCs from influenza-infected mice).

  • c Two rows are shown for each hybrid, representing duplicate experiments selected from a total of three to four.

  • d Numbers in parentheses refer to the increase in frequency relative to the unsorted population. Thus, numbers <1 represent a depletion in the APC frequency, and numbers >1 represent an enrichment.