Table I.

Infiltration of islets posttransfer of Th1 or Th2 cells to NOD.scid recipients

CD4 SubsetaNo InfiltratePeri-insulitisInsulitisTotal Islets
Th1 (n = 6)50 (30%)80 (49%)34 (21%)164
Th2 (n = 6)146 (91%)11 (7%)4 (2%)161
p value Student’s t test (paired)<0.0010.0360.009
  • a Th1 and Th2 cells were generated from CD4 cells that were isolated from the spleens of BDC 2.5 TCR transgenic mice by culture in anti-CD3-coated flasks in medium supplemented with rIL-2 and anti-CD28 and either rIL-12 and anti-IL-4, or rIL-4 and anti-IFN-γ, respectively, as described in Materials and Methods. After 4 days, 8 × 106 cells of each subset were transferred to groups of NOD.scid recipients (n = 6). On day 3 after cell transfer, paraffin-embedded sections of the pancreas were stained with hematoxylin and eosin, and individual islets were scored for the presence of infiltrates. Between 10 and 30 islets per pancreas were analyzed in five levels of sections that differed by 80 μm. For each category, the two groups of recipients were significantly different as calculated using the Student t test (paired).