Table II.

Effect of methylation of LVS DNA on protection against lethal F. tularensis LVS infection

DNA i.m. Day 0aDeaths/Total
Expt. 1Expt. 2Expt. 3Expt. 4
PBS5/55/54/55/5
2 μg LVS DNA1/50/51/50/5
2 μg Me-LVS DNA4/53/54/54/5
  • a BALB/cByJ mice (groups of five) were treated and challenged on day 3 after DNA treatment with 104 LVS i.p.; actual priming and challenge doses were confirmed by plate count at the time of inoculation. Mice were observed for morbidity and mortality through day 30, at which time surviving mice were sacrificed to assess clearance of bacteria from spleens. Time to death of those mice that died ranged from 4 to 8 days after challenge, and no group had a significantly different MTD from the matched PBS-treated control mice.