Table II.

Disease severity of adoptively transferred EAE is reduced by in vivo treatment with soluble OX-40R:Ig

Number of Cells TransferredaAnimal NumberTreatmentbTreatment Regimenc (day of disease)Max. Disease Severity Score10-day Cumulative Disease ScoredEAE Deaths
Expt. A
5× 106n = 4OX-40R:Ig (150 μg/day)1, 3, 55.031 ± 14.9g0/4
5 × 106n = 4Hu IgG (150 μg/day)1, 3, 57.549.5 ± 12.80/4
Expt. B
5× 106n = 5OX-40R:Ig (150 μg/day)1, 2, 3, 4, 55.40/5
5× 106n = 5Hu IgG (150 μg/day)1, 2, 3, 4, 57.6NAe2/5f
  • a EAE was adoptively induced by the transfer of PLP139–151-specific T cell lines, after activation with specific peptide (5 μg/ml) and syngeneic irradiated thymocytes.

  • b OX-40R:Ig and Hu IgG were diluted in sterile saline at the appropriate concentration to deliver the indicated amount in 0.2 ml of saline (i.p. injection).

  • c At the first sign of adoptively induced EAE, animals were paired according to clinical disease score and then split equally between the two treatment groups. Groups were then treated as indicated.

  • d The 10-day cumulative disease score is determined by adding the daily clinical disease score of an individual animal for the first 10 days of disease.

  • e Not applicable because two animals did not survive to day 10 of disease.

  • f Animals died of disease despite receiving gelatin squares and daily injections of saline as a diluent of Hu IgG.

  • g Significant relative to Hu IgG treatment group (p = 0.03), as determined by Student’s t test.