Table IV.

Rate and affinity constants of VpreB and scSL to different proteins coupled to the dextran matrixa

Soluble AnalyteImmobilized VpreBImmobilized scSLImmobilized VH-CH1 Nalm6Immobilized VH-CH1 1E8
konbkoffKdkonkoffKdkonkoffKdkonkoffKd
VpreB4.29 × 1041.08 × 10−32.52 × 10−83.10 × 1040.99 × 10−33.20 × 10−83.60 × 1041.14 × 10−33.16 × 10−84.57 × 1041.15 × 10−32.51 × 10−8
scSL3.82 × 1043.03 × 10−47.90 × 10−83.64 × 1055.54 × 10−41.52 × 10−93.45 × 1054.10 × 10−41.19 × 10−93.12 × 1058.38 × 10−52.68 × 10−10
  • a Six serial dilutions (400:12.5 nM) of VpreB or scSL were injected over four surfaces containing 700 RU, 410 RU, 870 RU, and 970 RU of VpreB, scSL, VH-CH1 Nalm6, and VH-CH1 1E8, respectively. The BIAcore Kinetics Evaluation program was used for data analysis.

  • b kon, association rate constant (M−1s−1); koff, dissociation rate constant(s−1); Kd = k off/kon, apparent dissociation constant (M).