Table I.

Recognition of swine and avian influenza A virus-infected target cells by human T cell lines

Cell LineaCD4/CD8ProteinEpitopesb (amino acids)Subtype SpecificityHLA Restriction% Specific 51Cr Release of Target Cells Infected withc
Expt. AExpt. BExpt. C
A/PR/8/34 (H1N1)A/NJ/1/76 (Hsw1N1)A/Duck/Pen/84 (H5N2)A/Duck/Alb/76 (AvH1N1)A/HK/156/97 (H5N1)A/HK/483/97 (H5N1)
Donor 1
1-1CD8NP383 –391H1, H2, H3B2717.013.28.026.548.520.4
1-7-KCD8M158 –66H1, H2, H3A2.145.631.443.542.564.957.9
10-2C2CD8NS1122 –130H1, H2, H3A2.120.015.1−9.918.74.6−1.3
10-1C4CD8NP174 –184H1, H2, H3B2710.15.4−4.03.413.76.3
10-1G5CD8HAH1, H2B1826.87.23.310.041.918.8
10-1B7CD8PB2H1, H2, H3B2743.88.714.330.761.355.4
1-2F8CD8PB1H1, H2, H3B2721.110.112.413.041.915.7
1-3CD4M117 –31H1, H2, H3DR165.665.219.120.333.12.6
10D9-d10CD4NAH1DR137.5−3.1−10.5−1.87.9−2.5
Donor 2
3G11CD8NP315 –496H1, H2, H3B6226.741.773.665.579.784.0
3E5CD4NP254 –262H1, H2, H376.977.980.869.482.275.1
3E9CD4NAH114.13.2−1.253.714.49.7
10E7CD4M1H1, H2, H324.521.850.049.883.379.6
Donor 3
124CD8M27 –15H1, H2B4426.82.6−0.81.5−2.8−0.7
77CD4NS134 –42H1, H2, H3DR379.171.33.873.868.565.4
  • a Each donor was tested separately in three experiments.

  • b —, HLA restriction or epitope not yet defined.

  • c These results were obtained using PBMC of American donors; although the frequency of HLA alleles varies considerably between distinct population groups, some, e.g., HLA A2, represent ∼30% of both North American Caucasian and Southern Han, the dominant tribe in southern China (31). E:T ratio was 10:1–20:1 and uninfected targets were used as a negative control with lysis <1% by all of the T cell lines.