Table I.

Cellular activation and proliferation of allostimulated PBMCsa

% CD4+/CD69+% CD4+/HLA-DR+% CD4+/CCR5+% CD4+/CXCR4+[3H]Thymidine Incorporation
Donor 10.701.708.54 [16]69.75 [57]233 cpm
Donor 20.662.252.54 [14]76.39 [61]145
Donors 1 + 28.009.371.67 [13]89.07 [64]7980
  • a Unfractionated PBMCs from two donors were unstimulated (donor 1 and donor 2) or allostimulated with each other (donors 1 + 2) for 4 days, and expression of cellular activation markers (CD69, HLA-DR) and HIV-1 coreceptors (CCR5, CXCR4) were determined for CD4+ T cell subpopulation by FACS. The percentages of CD3+, CD19+, and CD14+ subsets in the culture were 78, 15, and 4%, respectively. The mean fluorescence intensity (MFI) of CCR5 or CXCR4 staining is shown in parentheses. MFI for isotype control was 12 and 11, respectively. [3H]Thymidine incorporation by 104 PBMCs was also measured. Results are representative of five independent experiments.