Table I.

Donor cell populations after neutrophil enrichment of peripheral blood leukocytesa

DonorCellular CompositionbViability at 12 h (%)c
NeutrophilsEosinophilsMonocytesLymphocytesMediaSTAg
1260.0 ± 18.4 (70%)88.0 ± 25.6 (24%)5.0 ± 0 (1%)17.0 ± 8.5 (5%)9694
2231.0 ± 65.1 (92.4%)6.0 ± 2.8 (2.4%)4.5 ± 2.1 (1.8%)8.5 ± 0.7 (3.4%)9391
3267.3 ± 32.5 (91.2%)16.7 ± 3.1 (5.3%)1.7 ± 1.5 (0.3%)12.6 ± 2.5 (3.3%)9796
4288.1 ± 1.4 (96%)9.0 ± 1.4(3%)0 (0%)3.0 ± 1.5 (1%)9496
  • a PMN were isolated from each individual by centrifugation over a Percoll gradient as described in Materials and Methods.

  • b Cellular composition was determined visually after staining cytospin preparations with Diff-Quik.

  • c Viability was determined by trypan blue exclusion.