Table II.

Conjugate formation between Ly49A+ RMA cells and Con A blasts from B6, D8, and B6DP micea

Con A BlastNo mAbbControl mAbAnti-Ly49A mAbc
B67.1 ± 3.14.5 ± 2.67.1 ± 2.7
D8 18.3 ± 3.8 17.6 ± 7.0 9.8 ± 4.8
B6DP 14.2 ± 3.9d 12.2 ± 5.7 7.4 ± 2.5
  • a Mean percentage of conjugates (± SD) between Ly49A-expressing RMA cells and Con A blasts in absence or presence of anti-Ly49A mAb or isotype-matched control mAb (anti-H-2Ld). The anti-Ly49A-blocking data are derived from nine separate experiments. The control mAb was included in two of these experiments.

  • b Values statistically different (p < 0.05) from B6 Con A blasts in the absence of mAb are shown in bold. Statistically significant differences were assessed using paired Student’s t test.

  • c Values statistically different (p < 0.05) from Con A blasts in the absence of mAb are shown in bold. Statistically significant differences were assessed using paired Student’s t test.

  • d Significantly (p < 0.05) fewer number of conjugates formed with B6DP compared with D8 Con A blasts. Statistically significant differences were assessed using paired Student’s t test.